Fig. 2

Expression pattern of Nrg1 in the olfactory bulb. A Diagram of mouse brain sagittal section (top) and coronal section (bottom). The line in the sagittal section diagram indicates the position of the coronal section. B Fluorescent images of tdTomato, Gad67-GFP and NeuN in the OB of 2-month-old Nrg1-reporting mice. D, dorsal. M, medial. Scale bar, 300 μm. C The percentage of Nrg1-positive cells in different layers among total Nrg1-positive cells in the OB. P < 0.0001, n = 3 mice (9 slices), one-way-ANOVA. D Expression of tdTomato after injection of Cre-reporting AAV into the GCL of 2-month-old Nrg1^Cre/+ mice. Four weeks after AAV injection, the brain slices were collected and analyzed. Scale bar, 300 μm. E Fluorescent images of tdTomato, Gad67-GFP and NeuN in the GCL, from the rectangle E in panel B. Arrows indicate Nrg1-positive granular cells in the GCL. Scale bar, 50 μm. F Fluorescent images of tdTomato, Gad67-GFP and NeuN in the ML, from the rectangle F in panel B. Arrows indicate Nrg1-positive GABAergic interneurons in the ML. Scale bar, 50 μm. G Fluorescent images of tdTomato, Gad67-GFP, TH and DAPI in GL. Arrows indicate Nrg1-positive PG cells expressing Gad67-GFP but not TH. Scale bar, 50 μm. The Nrg1-reporting mice were crossed with Gad67-GFP mice to visualize the expression of Nrg1 in GABAergic interneurons. ONL: olfactory nerve layer; GL: glomerular layer; EPL: external plexiform layer; ML: mitral cell layer; IPL: internal plexiform layer; GCL: granular cell layer; GrA: granule cell layer of the accessory olfactory bulb; RMS; rostral migratory stream