Fig. 6

Zer-loaded nanoemulsion inhibits UVA-induced skin aging. (a) Schematic of the preparation of Zer-loaded nanoemulsion (NE-Zer). (b) Representative TEM image of the Zer-loaded nanoemulsion. (c) Particle size distribution of the Zer-loaded nanoemulsion as determined via DLS. (d and e) Antera 3D plots of WT and Tagln^−/− mice treated with Zer or NE-Zer. The wrinkle score and maximum depth are shown in the right panel. (f) Representative H&E-stained skin sections in each group of WT and Tagln^−/− mice with Zer or NE-Zer treatment before UV irradiation. (g) Masson staining of collagen (blue) in the skin sections of WT and Tagln^−/− mice with Zer or NE-Zer treatment before UV irradiation. (h and i) IHC analyses of MMP1 and COL1A2 in the skin sections of WT or Tagln^−/− mice with Zer or NE-Zer treatment before UV irradiation (h). Scale bar: 200 μm. Representative images and staining scores are shown (i). (j–l) Hyp (j), MDA (k), and SOD (l) levels in WT and Tagln^−/− mice with Zer or NE-Zer treatment before UV irradiation. All values are expressed as mean ± SD, n.s, not significant, ^*P < 0.05, ^**P < 0.01