Fig. 5
From: Interaction of TAGLN and USP1 promotes ZEB1 ubiquitination degradation in UV-induced skin photoaging
Zer targets the TAGLN/USP1 interface and promotes USP1 binding to ZEB1. (a) 2D diagram of the ligand interaction of Alb, Zer, or PDL with TAGLN. (b) Effects of Alb, Zer, and PDL on cell viability detected by CCK-8 assay. (c) Effects of Alb, Zer, and PDL on UV-induced cell damage examined by CCK-8 assay. (d–f) SOD (d), MDA (e), and ROS (f) levels of HSFs under Alb, Zer, or PDL pre-protection before UVA irradiation. g) Predicted binding modes of Zer and the TAGLN/USP1 complex. h) Effect of Zer on ZEB1 ubiquitination levels. i) Western blot analyses of ZEB1, P53, MMP1, and COL1A2 in HSFs treated with UVA and different Zer concentrations. j) Interaction of ZEB1 or TAGLN with USP1 in HSFs treated with 5 or 10 µM Zer as detected by Duolink PLA. All values are expressed as mean ± SD, n.s, not significant, *P < 0.05, **P < 0.01 compared with the UV-treated group. #P < 0.05, ##P < 0.01 compared with the control group