Fig. 4
From: Interaction of TAGLN and USP1 promotes ZEB1 ubiquitination degradation in UV-induced skin photoaging
USP1 stabilizes ZEB1 by deubiquitination. (a) Statistical analysis of the correlation between USP1 and ZEB1 expression in human skin samples from the GEPIA database (P < 0.05, R = 0.3). (b) Detailed binding mode of USP1 (blue) and ZEB1 (green). (c) Effect of USP1 overexpression on ZEB1 expression level as detected by Western blot. (d) Effect of USP1 or ZEB1 knockdown on each other expression level as analyzed by Western Blot. (e) HSFs were transfected with control siRNA or USP1 siRNA with or without 50 µM MG132 and then subjected to Western blot analysis using anti-USP1 and ZEB1 antibody. (f) Cycloheximide chasing experiment showing ZEB1 stability after USP1 overexpression or knockdown. (g) Effects of USP1 on ZEB1 ubiquitination levels. All values are expressed as mean ± SD, n.s, not significant, *P < 0.05, **P < 0.01