Fig. 3
From: Interaction of TAGLN and USP1 promotes ZEB1 ubiquitination degradation in UV-induced skin photoaging
TAGLN reduces the interaction between ZEB1 and USP1 by competitive interaction with USP1. (a) PPI enrichment map of the intersection of the respective interacting proteins of TAGLN and ZEB1. (b) Venn diagram of the DUBs interacting with TAGLN or ZEB1. (c) Pull-down and mass spectrometry analyses of TAGLN- (left) and ZEB1-associated proteins (right). (d) Whole-cell lysates from HSFs immunoprecipitated and immunoblotted with antibodies against the indicated proteins. (e) TAGLN and USP1 expression levels in HSFs following USP1 overexpression or knockdown as determined by Western blot. (f) Interaction of ZEB1 or TAGLN with USP1 in UVA-induced HSFs as detected via Duolink PLA. (g) Cytosolic and nuclear proteins extracted from HSFs after 10 J/cm2 UV irradiation and subjected to the Western blot analyses of TAGLN, ZEB1, and USP1. (h) IF showing the locations of TAGLN and USP1 in HSFs treated with UVA or UVA + cytochalasin B. (i) Effects of TAGLN expression level on ZEB1 ubiquitination levels as shown by Western blot. All values are expressed as mean ± SD, n.s, not significant, *P < 0.05, **P < 0.01