Fig. 5

Microglia degrades Tau deposits by actin remodeling, localized with TKS5 and Arp2. A Microglia degraded Tau deposits through the accumulation of podosome and filopodia, which were localized with Arp2 actin nucleator at the site of degradation (Scale bar 10 μm). The arrow indicates the degradation area. B The actin remodeling is mediated by Arp2, where filopodia contained more Arp2 than podosome, relating to rapid actin polymerization at the Tau deposits degradation site (No. of experiment = 3) (n = 30). C Similarly, the TKS5 adaptor protein became colocalized with podosome and filopodia at the site of Tau deposits degradation (scale bar 10 μm). The arrow indicates the degradation area. D. But, the colocalization of F-actin and TKS5 did not alter between podosome and filopodia-associated Tau deposits degradation. (No. of experiment = 3) (n = 30) E Tau fluorescence intensity was significantly reduced in the degradation area of Tau monomer and oligomers-deposits as compared to the non-degraded area. Moreover, Tau monomer was significantly degraded more than oligomers in microglia-mediated deposit degradation (No. of experiment = 3) (n = 100). F The quantification of relative degraded area/total cell area revealed that monomer deposits were better degraded by microglia as compared to Tau oligomers deposits. (No. of experiment = 3) (n = 45). Hence, It can be concluded that microglia prefer to degrade Tau monomer more than oligomers as deposits as emphasized by Time and area of degradation