Fig. 1

MXC dampens intracellular DNA- but not RNA-sensing pathway. a L929 cells were seeded in 96-well plates and cultured in the presence of MXC at indicated concentrations for 24 h and CC₅₀ was calculated from the curve after nonlinear regression analysis. b L929 cells were pretreated with different concentrations of MXC for 6 h and then treated with 0.5 µg/ml HT-DNA for 3 h. The mRNA level of Ifnb was analyzed by qPCR and IC₅₀ was calculated from the curve after nonlinear regression analysis. c, d qPCR analysis of Ifnb mRNA expression in L929 cells treated with HT-DNA (0.5 µg/ml) (c) or Poly(I:C) (1 µg/ml) (d) for 3 h following a 6 h of pretreatment with 500 µM MXC. e Representative fluorescent images of L929 cells transfected with Cy5-labeled DNA (1 µg/ml) for 3 h following a 6 h of pretreatment with 500 µM MXC. Scale bar, 50 μm. f Relative fluorescence of Cy5-labeled DNA per cell was quantified from 25 views, n = 3 biological replicates. g Representative fluorescent images of L929 cells transfected with Cy5-labeled RNA (1 µg/ml) for 3 h following a 6 h of pretreatment with 500 µM MXC. Scale bar, 50 μm. h Relative fluorescence of Cy5-labeled RNA per cell was quantified from 25 views, n = 3 biological replicates. Data are presented as the mean ± s.e.m. ***P < 0.001. Data represent at least three biological independent experiments