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Fig. 3 | Cell & Bioscience

Fig. 3

From: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1

Fig. 3

Isg15 deficiency impacts ovulation in mice. A Illustration of the hormonal injection strategy in mice. B H&E staining of ovarian sections from three-week-old Isg15+/+ and Isg15−/− mice stimulated by PMSG/hCG. AF: antral follicle. Pre: pre-ovulatory follicle. CL: corpora lutea. Black triangles show ruptured follicles. Scale bar: 200 μm. C Forty-four hours after PMSG injection, the number of antral follicles was counted in Isg15+/+ and Isg15−/− ovaries. D Ten hours after PMSG/hCG injection, the number of pre-ovulatory follicles was counted in Isg15+/+ and Isg15−/− ovaries. E Sixteen hours after PMSG/hCG injection, the number of ruptured follicles was counted in Isg15+/+ and Isg15−/− ovaries. F Twenty-four hours after PMSG/hCG injection, the number of corpora lutea was counted in Isg15+/+ and Isg15−/− ovaries. G The number of oocytes ovulated by Isg15+/+ and Isg15−/− mice after superovulation treatment (n = 6). H Cumulus-oocyte complexes (COCs) isolated from Isg15+/+ and Isg15−/− mice were cultured in vitro for 0 h, 10 h, 16 h. Scale bar: 20 μm. I Cumulus expansion of COCs from Isg15+/+ and Isg15−/− mice was scored after 16 h in vitro culture (n = 50 for each group). J, K Expression of cumulus expansion-linked genes including Adamts1, Ptgs2, Has2, Runx2, Tnfaip6, Ptx3 were assessed by quantitative RT-PCR (J) and western blot (K) in the cumulus cells from Isg15+/+ and Isg15−/− mice. All data are mean ± SD; *P < 0.05

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