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Fig. 1 | Cell & Bioscience

Fig. 1

From: ISG15 suppresses ovulation and female fertility by ISGylating ADAMTS1

Fig. 1

Isg15 deletion improves female fertility in mice. A The schematic diagram of constructing an Isg15 knockout mouse model. B Quantitative RT-PCR analysis of Isg15 in ovaries of Isg15+/+ and Isg15−/− mice. C Western blot analysis of ISG15 in ovaries of Isg15+/+ and Isg15−/− mice. The right bar graph showed the quantification of protein level. D Immunofluorescence analysis of ISG15 in ovaries of Isg15+/+ and Isg15−/− mice. Red: ISG15; Blue: DAPI; Scale bars: 500 μm. E Cumulating number of pups born per Isg15+/+ (blue line) and Isg15−/− (red line) female mice when bred with Isg15+/+ male mice during the eight-month period of fertility test (n = 6). F Embryos in Isg15+/+ and Isg15−/− mice. The right panel is the number of embryos. G Sixteen hours after natural mating, oocytes were collected from Isg15+/+ and Isg15−/− oviducts. The rightmost panel is the number of oocytes. Scale bar: 20 μm. H Estrus cycle of eight-week-old Isg15+/+ and Isg15−/− mice. I, J Serum FSH (I) and LH (J) levels in eight-week-old Isg15+/+ and Isg15−/− mice. All data are mean ± SD; *P < 0.05, ***P < 0.001

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