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Fig. 3 | Cell & Bioscience

Fig. 3

From: FACI is a novel clathrin adaptor protein 2-binding protein that facilitates low-density lipoprotein endocytosis

Fig. 3

DxxxLI motif of FACI is required for its localization to CCPs. A Multiple alignments of FACI and homologs. Conserved Motifs B (YxxL), D (DxxxLI) and E (PIP2-binding motif) were boxed and labeled. FACI homologs were from elephant shark (sb:cb1058, XP_042201215.1), zebrafish (sb:cb1058, NP_001373652.1), two-lined caecilian (LOC115096979, XP_029468198.1), green sea turtle (C11orf86 homolog, XP_027674344.2), koala (C11orf86 homolog, XP_020824020.1) and human (C11orf86, NP_001129957.1). B Protein sequences of FACI and its mutants. Motifs B (YxxL), D (DxxxLI) and E (PIP2-binding motif) were highlighted. The amphipathic α-helix of Motif E was represented as a poly-H cluster. C TIRFM images of AML12 cells expressing mCherry-AP2M1 and mEmerald-FACI mutants (FACI-ΔYxxL, FACI-ΔDxxxLI, and FACI-ΔYxxL-DxxxLI). Scale bar, 5 µm. D TIRFM images of AML12 cells expressing mCherry-AP2M1 and mEmerald-FACI mutants. FACI-Δ2-68 contains Motifs D and E of FACI, while FACI-Δ2-82 just retains Motif E. PIP2: phosphatidylinositol 4,5-bisphosphate. Scale bar, 5 µm

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