Fig. 5

FZD7 attenuates the BCL6-induced ferroptosis facilitation of GC cells. A, B AGS and SGC-7901 cells were transfected with control vector or BCL6 plasmid. After 6 h, cells were seeded in 96-well plates overnight. Cell death was detected by MTT assay for cells treated with or without corresponding concentration of erastin and RSL3 after 48 h (***p < 0.001). C, D, E Lipid peroxidation (Lipid ROS), lipid Oxidation (MDA) and Fe²⁺ level were measured in AGS and SGC-7901 cells transfected with control vector or BCL6 plasmid (***p < 0.001). F, G AGS and SGC-7901 cells were transfected with control vector (Ctrl1 + Ctrl2, Ctrl1 + FZD7, BCL6 + Ctrl2) or co-transfected BCL6 plasmid and FZD7 plasmid (BCL6 + FZD7). Then, cells were treated as in A, B, and cell death was detected (***p < 0.001). H, I, J Lipid ROS, MDA and Fe²⁺ level were measured in AGS and SGC-7901 cells transfected with control vector (Ctrl1 + Ctrl2, Ctrl1 + FZD7, BCL6 + Ctrl2) or co-transfected BCL6 plasmid and FZD7 plasmid (BCL6 + FZD7) (***p < 0.001)