Fig. 4
BCL6 exerts its tumor suppressor function by repressing FZD7. A CCK8 assay showed that FZD7 overexpression reversed BCL6-inhibited growth in AGS and SGC-7901 cells (**p < 0.01, ***p < 0.001). B Colony formation assay showed that FZD7 overexpression reversed BCL6-inhibited colony formation in AGS and SGC-7901 cells (**p < 0.01, ***p < 0.001). C, D FZD7 overexpression attenuated the inhibition of BCL6 on cell invasion and migration, as measured by wound healing and transwell assay (Scale bar, 100 μm) (**p < 0.01, ***p < 0.001). E Immunofluorescence staining for β-catenin (red) and 4′,6-diamidino-2-phenylindole (DAPI) (blue) in AGS and SGC-7901 cells transfected with control vector (Ctrl1 + Ctrl2, Ctrl1 + FZD7, BCL6 + Ctrl2) or co-transfected BCL6 plasmid and FZD7 plasmid (BCL6 + FZD7) (Original magnification, × 400)