Fig. 4
From: Slit-Robo expression in the leech nervous system: insights into eyespot evolution
Immunostaining for acetyl-tubulin (ac-tubulin) provides landmarks for the regions within the segmental ganglia where Hau-slit and Hau-robo1 are expressed. a: Ventral view showing ac-tubulin staining of the CNS. a': Cross section of stage 10 embryo showing ac-tubulin staining in neuropil (open arrowhead) and commissures (white arrowhead). b: Ventral view showing Hau-slit expression. b': Cross section of stage 10 embryo showing Hau-slit staining neuropil partially. c: Merged image showing Hau-slit transcripts overlapping partially with commissures (white arrowhead). c': Merged image of both cross sections showing Hau-slit transcripts at the ventral and dorsal margins of the neuropil (open arrowhead). d: Ventral view showing ac-tubulin staining of the CNS. d': Cross section of stage 10 embryo showing ac-tubulin staining in neuropil and commissures. e: Ventral view showing Hau-robo1 expression. e’: Cross section of stage 10 embryo showing Hau-robo1 staining. f: Merged image showing Hau-robo1 transcripts surrounding every ganglia and overlapping partially with the segmental nerves, commissures, neuropil and the connective. f': Merged image of both cross sections showing Hau-robo1 transcripts along the midline in between each hemiganglia and overlapping partially with commissures, neuropil and the connective. Anterior is up in a-c and d-f; dorsal is up in a'-c' and d'-f'. Scale bar: 100 μm in a-f; 50 μm in a'-f'