Fig. 7
From: RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells
Apoptosis levels in the germ cells of the Amh-Rnf20−/− testes were increased significantly during spermatogenesis. a, b Comparison of the number of spermatocytes (a) and spermatogonia (b) in the seminiferous tubules between the Rnf20Flox/Flox and the Amh-Rnf20−/− in adults and at 7, 14, 16, 18, 21, and 28 days after birth. Y axis, the number of germ cells; X axis, postnatal days. Black dots on the lines represent the Rnf20Flox/Flox, black squares on the lines represent the Amh-Rnf20−/−. *, p < 0.05, **, p < 0.01. c Western blot analysis of the expression levels of RNF20 and Caspase3 in testes of adult mice of the Rnf20Flox/Flox and the Amh-Rnf20−/−. β-ACTIN was used as an internal control. d TUNEL assays were used to analyze the apoptotic signals in testes of the Rnf20Flox/Flox and the Amh-Rnf20−/− in adults and at 7, 14, 16, 18, 21, and 28 days after birth. The nuclei were stained with DAPI. The green fluorescence showed the apoptosis signals. The white squares in the panels correspond to the enlarged panels. Scale bar, 75 μm. e Statistical analysis of the proportion of TUNEL-positive seminiferous tubules. *, p < 0.05, **, p < 0.01. f Statistical analysis of the cell number with TUNEL-positive signals in the seminiferous tubules. Black dots represent the Rnf20Flox/Flox, blue squares indicate the Amh-Rnf20−/−. *, p < 0.05, **, p < 0.01