Fig. 3
From: RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells
RNF20 deficiency in Sertoli cells impairs the Cldn11 transcription. a Scatterplots of differentially expressed genes. Red scatter, genes with significant up-regulated; blue scatter, genes with significant down-regulated; gray scatter, genes with no significant difference. X axis, Lg (WT FPKM) in the Rnf20Flox/Flox; Y axis, Lg (KO FPKM) in the Amh-Rnf20−/−. b Gene ontology (GO) terms analysis of down-regulated genes in the Sertoli cells of the Amh-Rnf20−/− testes. c, d Heatmaps showing the expression levels of down-regulated genes in the terms spermatogenesis (c) and cell adhesion (d) in the Sertoli cells of the Rnf20Flox/Flox and the Amh-Rnf20−/−. Color bar, Log2 (FPKM). e Quantitative real-time PCR analysis of the genes Rnf20 and Cldn11. The expression levels of the genes were related to Hprt expression. Relative levels, 2−ΔCt; T-tests were performed. *, p < 0.05, **, p < 0.01. f Western blot analysis of the expression levels of RNF20, CLDN11, and H2BK120ub proteins in adult mice. β-ACTIN was used as an internal control. g ChIP-PCR assays. The antibody specific for H2BK120ub was used in the ChIP analysis and primers were designed in the regions of promoter and exons of Cldn11 in the testes of the Rnf20Flox/Flox and the Amh-Rnf20−/−. The black graphs indicated the enriched levels in the Rnf20Flox/Flox mice, while the white graphs indicated the levels in the Amh-Rnf20−/− mice