Fig. 1
From: RNF20 is required for male fertility through regulation of H2B ubiquitination in the Sertoli cells
Spermatogenic failure in the Amh-Rnf20−/− mice. a Schematic diagram for the construction for making the Rnf20Flox/Flox and the Amh-Cre mice. b Flow chart of breeding to generate the Amh-Rnf20−/− mice. c Genotyping of the Rnf20 conditional knockout mice. Rnf20-Flox, Rnf20Flox/Flox; WT, wild type; Amh-Cre, Amh promoter-induced conditional knockout. d Western blot analysis of the protein levels of RNF20 and the ubiquitylation of histone H2BK120 in adult mice of 3 types of genotypes. β-ACTIN was used as an internal control. e Comparison the size of testes between the Rnf20Flox/Flox and the Amh-Rnf20−/− in adult and at 7, 14, 16, 18, 21, and 28 days after birth. Scale bar, 100 μm. f Relative weight of testis to body weight in mice between genotypes of the Rnf20Flox/Flox and the Amh-Rnf20−/− (n = 3), *, p < 0.05, **, p < 0.01. g H&E staining of testes in the Rnf20Flox/Flox and the Amh-Rnf20−/− in adult and at 7, 14, 16, 18, 21, and 28 days after birth. The black square in the panels corresponds to the enlarged panels. Scale bar, 100 μm. h H&E staining of epididymides in adult mice. Scale bar, 100 μm