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Fig. 5 | Cell & Bioscience

Fig. 5

From: PSMD2 contributes to the progression of esophageal squamous cell carcinoma by repressing autophagy

Fig. 5

The involvement of ASS1 in the proliferation promoted by PSMD2. A, B The impact of ASS1 knockdown on the proliferation of KYSE30 cells (A) and KYSE450 cells (B) with PSMD2 overexpression. The cells were fluorescently stained with EdU (red). The nucleus was stained with DAPI (blue). The percentage of EdU-positive cells was calculated and shown in the right panel. Scale bar: 100 μm. C, D The impact of ASS1 knockdown on the proliferation of KYSE30 cells (C) and KYSE450 cells (D) with PSMD2 overexpression. E, F The impact of ASS1 overexpression on the proliferation of KYSE30 cells (E) and KYSE450 cells (F) with PSMD2 knockdown. The cells were fluorescently stained with EdU (red). The nucleus was stained with DAPI (blue). The percentage of EdU-positive cells was shown in the right panel. N = 3. G-H The impact of ASS1 overexpression on the proliferation of KYSE30 cells (G) and KYSE450 cells (H) with PSMD2 depletion. N = 3. Data were presented as mean ± SD, ***p < 0.001, and****p < 0.0001

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