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Fig. 4 | Cell & Bioscience

Fig. 4

From: PSMD2 contributes to the progression of esophageal squamous cell carcinoma by repressing autophagy

Fig. 4

The involvement of ASS1 in PSMD2-mediated inhibition of autophagy. A–D The impact of ASS1 knockdown on the formation of LC3B puncta in KYSE30 cells (A) and KYSE450 cells (B) with PSMD2 overexpression. The efficiency of ASS1 knockdown was shown in the right panel. Protein levels, upper right panel. RNA levels, bottom right panel. Autophagy was induced by FBS deprivation then stained with the DAPGreen (green). The nucleus was stained with Hoechst 33342 (blue). Scale bar: 30 μm. Quantitative analysis of the autophagic puncta of KYSE30 cells (C) and KYSE450 cells (D) was shown. N = 3.E–H The impact of ASS1 overexpression on the formation of LC3B puncta in KYSE30 cells (E) and KYSE450 cells (F) with PSMD2 knockdown. The treatment conditions were the same as those in (A, B). The efficiency of ASS1 overexpression was shown in the right panel. Protein levels, upper right panel. RNA levels, bottom right panel. Quantitative analysis of the autophagic puncta of KYSE30 cells (G) and KYSE450 cells (H) was shown. N = 3. Data were presented as mean ± SD, *p < 0.05, **p < 0.01, and****p < 0.0001

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