Fig. 4

The C/EBPα-BMP9 axis in the liver promotes APAP-ALI progression. APAP-ALI (300 mg/kg) was established 2 weeks after confirmation that Cebpa was successfully overexpressed by Cebpa-overexpressing AAV2/8 injection into the tail vein of the mice, and serum and liver tissue were collected from each group of mice after 24 h. A Serum ALT and AST levels. B Representative images showing H&E staining. C Representative images showing TUNEL staining images. D mRNA expression of Il1b, Il6 and Tnfa. E Representative images showing IHC staining for BMP9. F Representative images showing IHC staining for P-SMAD1/5/9. G Protein expression levels of C/EBPα, BMP9, SMAD1 and P-SMAD1/5/9. H Protein expression levels of ATG3, ATG7, LC3 I/II and p62. I Representative images showing IF staining for BMP9 and F4/80 in the liver slices of each group. The average target gene/Gapdh ratios different experimental groups relative to the control group. *p < 0.05, **p < 0.01, and ***p < 0.001