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Fig. 2 | Cell & Bioscience

Fig. 2

From: Aging aggravates acetaminophen-induced acute liver injury and inflammation through inordinate C/EBPα-BMP9 crosstalk

Fig. 2

BMP9 promotes hepatocyte injury and senescence. A, B mRNA (A) and protein (B) expression in the livers from young and aged mice. (CE) Primary hepatocytes with or without Rm-BMP9 addition were treated with APAP, and 1 h, 3 h, 6 h, 12 h and 24 h later, we measured the proportion of APAP-induced hepatocyte death to normal growing primary hepatocytes (C), the protein expression levels of SMAD1/5/9 and P-SMAD1/5/9 (D), and the protein expression levels of ATG3, ATG7, p62 and LC3 I/II (E). F, G Rm-BMP9 was injected into the tail vein, and the APAP-ALI model (300 mg/kg) was established 1 h later. Serum and liver tissue were collected from each group of mice 24 h after model establishment. We measured the serum ALT and AST levels (F), and representative images showing H&E staining (G) in each group are shown. H, I APAP-ALI model (300 mg/kg) was established with young and aged WT mice, as well as with young and aged Bmp9−/− mice. Serum and liver tissue were collected from each group of mice 24 h later. We measured the serum ALT and AST levels (H), and representative images showing H&E staining (I) in each group are shown. The average target gene/Gapdh ratios of different experimental groups to the control group are reported. *p < 0.05, **p < 0.01, and ***p < 0.001

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