Fig. 1

Aging accelerates APAP-ALI and inflammation. Young and aged mice were administered APAP (300 mg/kg) or PBS and analyzed 24 h later. A Serum ALT and AST levels of the young and aged mice treated with PBS or APAP. B Representative images showing H&E staining liver slices from young and aged mice treated with PBS or APAP. C Representative images showing TUNEL staining in the liver slices from young and aged mice treated with PBS or APAP. Quantitative data are shown in the right panel. D Representative images showing IHC staining for F4/80 in liver slices from young and aged mice treated with PBS or APAP. Quantitative data are shown in the right panel. E mRNA expression (Il1b, Il6, Tnfa, and Il10) in the livers from young and aged mice treated with PBS or APAP as measured by RT‒qPCR. The average target gene/Gapdh ratios of different experimental groups relative to the control group are reported. F Survival curves of young and aged mice treated with APAP when the dose of APAP was increased to 500 mg/kg. *p < 0.05, **p < 0.01, and ***p < 0.001