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Fig. 6 | Cell & Bioscience

Fig. 6

From: Exosomal USP13 derived from microvascular endothelial cells regulates immune microenvironment and improves functional recovery after spinal cord injury by stabilizing IκBα

Fig. 6

USP13 suppresses IκBα ubiquitination. a, b Evaluation and quantification of endogenous IκBα ubiquitination in indicated groups in BV2 microglia. ***P < 0.001. c, d Evaluation and quantification of exogenous IκBα ubiquitination in HEK 293T cells co-transfected with Flag-tagged USP13 (WT or C345A), HA-tagged Ub and Myc-tagged IκBα. ***P < 0.001. e, f Evaluation and quantification of IκBα ubiquitination in spinal cords of indicated groups. ***P < 0.001. g Evaluation of the IκBα ubiquitylation linkage in HEK 293T cells co-transfected with Flag-USP13, Myc-IκBα and the specific HA-Ub, Lys0, Lys48, or Lys63 plasmids. h Evaluation of IκBα and USP13 protein expression levels in HEK 293T cells transfected with Ub WT or Ub Lys48R in indicated groups

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