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Fig. 1 | Cell & Bioscience

Fig. 1

From: Exosomal USP13 derived from microvascular endothelial cells regulates immune microenvironment and improves functional recovery after spinal cord injury by stabilizing IκBα

Fig. 1

Exos treatment promotes M2 polarization and ameliorates mitochondrial impairment of LPS-pretreated microglia/macrophages in vitro. a Detection of mRNA expression of M1 and M2 markers in indicated groups. **P < 0.01, ***P < 0.001. b, c Detection and quantification of microglia M1 polarization by flow cytometry. ***P < 0.001. d, e Measurement and quantification of microglia M2 polarization by flow cytometry. ***P < 0.001. f, g Flow cytometry detection and quantification of ROS level. ***P < 0.001. h, i Detection and quantification of mitochondrial potential by JC-1 staining. ***P < 0.001. j, k Measurement of OCR and quantification of basal respiration, ATP production, respiratory capacity and respiratory reserve. **P < 0.01, ***P < 0.001

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