Fig. 1From: Exosomal USP13 derived from microvascular endothelial cells regulates immune microenvironment and improves functional recovery after spinal cord injury by stabilizing IκBαExos treatment promotes M2 polarization and ameliorates mitochondrial impairment of LPS-pretreated microglia/macrophages in vitro. a Detection of mRNA expression of M1 and M2 markers in indicated groups. **P < 0.01, ***P < 0.001. b, c Detection and quantification of microglia M1 polarization by flow cytometry. ***P < 0.001. d, e Measurement and quantification of microglia M2 polarization by flow cytometry. ***P < 0.001. f, g Flow cytometry detection and quantification of ROS level. ***P < 0.001. h, i Detection and quantification of mitochondrial potential by JC-1 staining. ***P < 0.001. j, k Measurement of OCR and quantification of basal respiration, ATP production, respiratory capacity and respiratory reserve. **P < 0.01, ***P < 0.001Back to article page