Fig. 6
HSF2BP interacts with CDC73 and regulates its subcellular localization. Liver is induced by 1-hour ischemia followed by 24-hour reperfusion in HSF2BP-KO and WT mice. Sham mice received all procedures except for hepatic ischemia. HL-7702 cells were transfected with HSF2BP overexpressed lentivirus (Lv-HSF2BP) or negative control lentivirus (Lv-NC) and treated under hypoxia/reoxygenation (H/R) condition (Culture under glucose/FBS free 1640 medium and hypoxia condition for 1 h, then transferred to glucose/FBS-rich medium and normoxia condition for 8 h). A, Western blot analysis of HSF2BP and CDC73 after coimmunoprecipitation in mice. Western blot analysis of CDC73 in the nucleus and cytoplasm (B) and their quantitative results (C, D) in mice. E, Western blot analysis of HSF2BP and CDC73 after coimmunoprecipitation in HL-7702 cells. Western blot analysis of CDC73 in the nucleus and cytoplasm (F) and their quantitative results (G, H) in HL-7702 cells. I, Immunofluorescent staining of HSF2BP and CDC73 in HL-7702 cells. Results are expressed as mean ± SE (n = 3–4/group) and compared by one-way ANOVA. * p < 0.05 versus control group, # p < 0.05 versus Lv-NC group. Western blot analysis of CDC73, GRP78 and p-IRE1α (J) and their quantitative results (K) in HL-7702 cells transfected with siRNA-CDC73. Results are expressed as mean ± SE (n = 3–4/group) and compared by one-way ANOVA. * p < 0.05 versus siRNA-NC group, # p < 0.05 versus Lv-NC-H/R group