Fig. 1

HSF2BP mitigates tunicamycin-induced ER stress. ER stress was induced by intraperitoneal injection of tunicamycin (0.5 µg/g body weight, 500 µl) for 48 h in mice. Sham mice treated with 500 µl normal saline. Liver H&E staining (A) and its histological score (B) in tunicamycin-induced ER stress in HSF2BP-TG and NTG mice. Original magnification, x100 and x200. C, Transmission electron microscope (TEM) of endoplasmic reticulum (ER) in tunicamycin-induced ER stress in HSF2BP-TG and NTG mice. Original magnification, x1.5k and x5.0k. Western blot analysis of ER stress-related proteins (G) and their quantitative results of GRP78 (D), p-IRE1α (E) and CHOP (F) in tunicamycin-induced ER stress in HSF2BP-TG and NTG mice. Results are expressed as mean ± SE (n = 4–6/group) and compared by one-way ANOVA. * p < 0.05 versus sham mice, ^# p < 0.05 versus NTG mice. The liver H&E staining (H) and its histological score (I) in tunicamycin-induced ER stress in HSF2BP-KO and WT mice. Original magnification, x100 and x200. Western blot analysis of GRP78, p-IRE1α and CHOP (J) and their quantitative results (K) in tunicamycin-induced ER stress in HSF2BP-KO and WT mice. Results are expressed as mean ± SE (n = 4–6/group) and compared by one-way ANOVA. * p < 0.05 versus sham mice, ^# p < 0.05 versus WT mice