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Fig. 4 | Cell & Bioscience

Fig. 4

From: N-methyl-d-aspartate receptors induce M1 polarization of macrophages: Feasibility of targeted imaging in inflammatory response in vivo

Fig. 4

Binding of NMDAR1-FSD Fluor™ 647 (N-TIP) to LPS-stimulated BMDMs. A BMDMs were treated with I-TIP or N-TIP (red), CellMask™ green (green), and Hoechst 33,342 (blue) for 3D-based imaging. B The white box in (A) is enlarged and the front and back images are shown. C The intensity of the 3D-based image was determined as the total NMDAR-FSD Fluor™ 647 area compared to the total CellMask™ green area. D–G Intact-BMDMs and LPS-stimulated BMDMs were incubated with either I-TIP or N-TIP, followed by FACS analysis. Flow cytometry histogram and bar graph to demonstrate the percentage of NMDAR on CD11b+ (D and E) or F4/80+ (F and G) macrophages. Length of X- and Y-axis: 160 μm, Z-axis: 14 μm. Data are expressed as the mean ± SD of three independent experiments. *p < 0.05, ***p < 0.005

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Cell & Bioscience

ISSN: 2045-3701

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