Fig. 2

M1 polarization of macrophages requires NMDAR-induced glycolysis. A and B Effects of Grin1-targeting siRNA (A) or MK-801 (1, 50, 150 μM; B) on phosphorylated PI3K, AKT, and p70S6K and HIF-1α and LDHA in LPS-treated BMDMs for 24 h. C Effects of rapamycin on the levels of iNOS, phosphorylated S6K, HIF-1α, and LDHA in LPS-treated BMDMs for 24 h. D Extracellular acidification rate (ECAR) in Grin1-silenced or/with Rapa (rapamycin)-treated BMDMs with LPS for 24 h. E, F Effects of LDHA-targeting siRNA on the levels of iNOS (E) and ECAR (F) in LPS-treated BMDMs for 24 h. Data are expressed as the mean ± SD of three independent experiments.. *p < 0.05, **p < 0.01, ***p < 0.005