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Fig. 5 | Cell & Bioscience

Fig. 5

From: TMED3 promotes the development of malignant melanoma by targeting CDCA8 and regulating PI3K/Akt pathway

Fig. 5

Elevating CDCA8 abolished the suppression of cell proliferation and migration from TMED3 depletion. A Celigo cell counting assay was performed to examine the effect of CDCA8 downregulation on A375 cell proliferation. B The ability of CDCA8-depleted A375 cells to form colonies was assessed. C The effect of CDCA8 downregulation on A375 cell migration was evaluated by wound-healing assay. D Flow cytometry was performed to visualize the changes of A375 cell apoptosis upon knocking down CDCA8. E–H The abilities of cell proliferation (E), colony formation (F), migration (G) and apoptosis (H) were assessed via CCK8 assay, colony formation assay, wound-healing experiment and flow cytometry analysis, respectively. NC (OE + KD): Control; CDCA8 + NC-shTMED3: CDCA8 overexpression; shTMED3 + NC-CDCA8: TMED3 downregulation; CDCA8 + shTMED3: CDCA8 overexpression combining with TMED3 downregulation. The data were expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001

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Cell & Bioscience

ISSN: 2045-3701

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