Fig. 2

TMED3 knockdown inhibited cell proliferation, colony formation and migration, induced cell apoptosis. A MTT assay was used to detect the effects of TMED3 knockdown on cell proliferation of A375 and OM431 cells. B The abilities of A375 and OM431 cells to form colony after infection were assessed. C The effects of TMED3 knockdown on A375 and OM431 cell migration capacities were detected by transwell assay. D The western blot was employed to detect the levels of EMT-associated elements in A375 and OM431 cells upon silencing TMED3. E Flow cytometry was performed to evaluate the effects of TMED3 knockdown on cell apoptosis of A375 and OM431 cells. F The changes in apoptosis-related proteins were analyzed in A375 cells following infection by a Human Apoptosis Antibody Array. Protein level was presented in gray value. The data were expressed as mean ± SD. * P < 0.05, ** P < 0.01, *** P < 0.001