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Fig. 5 | Cell & Bioscience

Fig. 5

From: GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint

Fig. 5

Downregulation of GPR116 receptor promotes the antitumor activity of NKG2D-CAR-NK92 cells in vitro. AC The line plots displayed the cytotoxicity of NK92 cells, NC-NKG2D-CAR-NK92 cells (normal control) and SH-NKG2D-CAR-NK92 cells (NKG2D-CAR-NK92 cells with GPR116 knockdown) against PANC28 cells, PANC1 cells and SW1990 cells at a different effector to target (E: T) ratios for 4 h. D Flow cytometry analysis of the expression of CD107a in NK92 cells after co-incubating with PANC28 cells at a 5:1 ratio for 4 h. E Flow cytometry analysis of the expression of GzmB in NK92 cells after co-incubating with PANC28 cells at a 5:1 ratio for 4. F The expression of HIF1α and p-P65 in NKG2D-CAR-NK92 cells by WB. Data are represented as the mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.001 by an unpaired Student’s t-test

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