Fig. 4

Deficiency of GPR116 receptor enhances the antitumor effect of NK cells in vivo. Subcutaneous tumor models were established with PAN02 cells and these mice were injected with PBS, WT-NK and GPR116^−/−-NK through tail vein, respectively. A The photo of PAAD tumor sizes (n = 4). B The PAAD tumor growth curves and the end-point tumor sizes were represented (n = 4). C The weight of PAAD tumor (n = 4). D The proportion of CD4⁺ and CD8⁺ T cells in PAAD tumor. E The infiltration of CD3⁻ NK1.1⁺ cells in PAAD tumor. F The expression of GzmB in tumor-infiltrating CD3⁻ NK1.1⁺ cells. G The expression of IFNγ in tumor-infiltrating CD3⁻ NK1.1⁺ cells. Data are represented as the mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01, ***P < 0.001 by an unpaired Student’s t-test