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Fig. 3 | Cell & Bioscience

Fig. 3

From: GPR116 receptor regulates the antitumor function of NK cells via Gαq/HIF1α/NF-κB signaling pathway as a potential immune checkpoint

Fig. 3

GPR116 receptor regulates the function of NK92 cells via HIF1α/NF-κB signaling pathway. A Western blot analysis of p-P65 and HIF1α in NK92 cells (NC) and GPR116-knockdown NK92 cells (SH). B Western blot analysis of p-P65 and HIF1α in NK92 cells (NC) and GPR116-knockdown NK92 cells (SH) with or without QNZ. C, D Western blot analysis of p-P65 and HIF1α in NK92 cells after the treatment with CoCl2. E Flow cytometry analysis of the killing ability of NK92 cells (NC) and GPR116-knockdown NK92 cells (SH) with or without QNZ. Flow cytometry analysis of the expression of GzmB (F), IFNγ (G) and CD107a (H) in NK92 cells (NC) and GPR116-knockdown NK92 cells (SH) with or without QNZ. All data are from at least three independent experiments. Data are represented as the mean ± standard error of the mean (SEM). *P < 0.05, **P < 0.01 by an unpaired Student’s t-test

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