Fig. 3

Distribution and formation of islets of Langerhans in the microenvironment of the pancreas. Microdissected pancreases of tdTomato reporter control-Ai14 and Isl1CKO-Ai14 mice were cleared (CUBIC protocol), immunolabeled, imaged, and reconstructed in 3D using light-sheet fluorescence microscopy (LFSM; see Additional files 2, 3, 4, 5: Videos S1–S4). LFSM images depict the distribution and formation of islets in the anatomical microenvironment of the pancreas at P9, showing tdTomato⁺ endocrine cell population and INS producing β cells together with anti-GLP1 labeled α cells in (a, b) or with neuronal fibers labeled by anti-TUBB3 in (c, d). Scale bars: 100 μm