Fig. 1

Experimental design of single RNA-seq on 3 staged colonic lesions in the same patient. A. Hematoxylin–eosin (H&E) staining of the tissues with different stages of colonic lesions including normal mucosa, colonic adenoma and carcinoma in the same patient. B. Overview of the experimental design of single cell RNA-seq. Pathological lesions of the colonic tissue including normal mucosa, adenoma and carcinoma were dissected and digested into single-cell suspensions for further separation, followed by library construction, sequencing, clustering and cell identification. C. tSNE plot of all the cells based on three different samples. D. tSNE plot of all the cells based on different cell types. E. KEGG enrichment of Top signal pathway in adenoma tissue compare to normal tissue, the blue bar and red bar show the down-regulated and up-regulated pathway, separately. F. KEGG enrichment of Top signal pathway in carcinoma tissue compare to normal tissue, the blue bar and red bar show the down-regulated and up-regulated pathway, separately. G KEGG enrichment of Top signal pathway in carcinoma tissue compare to adenoma tissue, the blue bar and red bar show the down-regulated and up-regulated pathway, separately