Fig. 3

RIPC fails to upregulate SIRT3 after NMDAR inhibition. A, B Immunofluorescence was used to detect the expression of NMDAR2B (in green) in neurons (in red) in different groups (n = 5/group). Nuclear was stained with DAPI (in blue). Scale bar = 100 μm. C Western blotting was used to detect the expression of NMDAR2B in different groups (n = 5/group). D, E The expression of SIRT3 (in green) in the spinal cord neurons (in red) was detected by immunofluorescence after administration of NMDR2B inhibitor MK-801 (n = 5/group). Scale bar = 100 μm. F The expression of SIRT3 in the spinal cord was detected by Western blotting after administration of MK-801 (n = 5/group). G Western blotting was used to detect the expression of SIRT3 at different time points after OGD/R (n = 5/group). H The expression of SIRT3 in neurons was detected by Western blotting after different treatment conditions and NMDA partially reversed the down-regulation of SIRT3 in neurons after OGD/R (n = 3/group). I The expression of SIRT3 in neurons was detected by Western blotting after different treatment conditions and sublethal glutamate partially reversed the down-regulation of SIRT3 in neurons after OGD/R (n = 5/group). Statistical analysis: mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001