Fig. 1

RIPC protects against SCIRI in mice. A Schematic diagram of the experimental design. B BMS scores at different time points post-injury in mice (n = 5/group). C Representative footprint images of mice on the third day following injury. Blue: frontpaw print; red: hindpaw print. D Quantification of the analysis of the footprint in each group (n = 5/group). E Representative images of MEP for assessing the electrophysiology of mice on the third day after injury. F Quantification of the peak-to-peak MEP amplitudes in each group (n = 5/group). G Representative images of Nissl staining of neurons in the anterior horn of the spinal cord. Scale bar = 100 μm. H Quantification of the number of integrated Nissl bodies per section (n = 5/group). I Representative images of TUNEL-positive apoptotic cells (in red) in spinal cord sections on day 3 post-injury. Neuron was stained with NeuN (in green) and nuclear stained with DAPI (in blue). Scale bar = 100 μm. J Quantification of the number of apoptotic cells in each group (n = 5/group). K MDA, MnSOD, CAT, and GSH were measured to reflected the level of oxidative stress in each group (n = 5/group). Statistical analysis: mean ± SEM, *p < 0.05, **p < 0.01, ***p < 0.001