Fig. 3
From: PABPN1 regulates mRNA alternative polyadenylation to inhibit bladder cancer progression
Upregulated PABPN1 leads to more transcripts with longer 3’UTRs in BC cells. A Scatter plot showing PDUIs of transcripts with significant (P < 0.05) lengthened (n = 712) or shortened (n = 115) 3’UTRs in PABPN1 overexpressing and control BC cells. B Volcano plot showing ΔPDUIPABPN1-Vector of transcripts generated by DaPars algorithm. ΔPDUIPABPN1-Vector = MeanPDUIPABPN1–MeanPDUIVector. C GO enrichment analysis of PABPN1 target genes with lengthened 3’UTRs. D Schematic illustration showing the PASs distribution of type a-c of PABPN1 target genes. E The number of genes in type a-c with lengthened or shortened 3’UTRs (left), the proportion of 3’UTR lengthened genes with or without non-canonical PAS in proximal 3’UTRs (right). F The flow chart for screening out potential targets of PABPN1 (left), the long transcript-to-total transcript ratio of potential target genes upon PABPN1 overexpression or knockdown measured by qRT-PCR (right). G Diagram showing luciferase reporters containing miRNA binding sites located between proximal and distal PASs in 3’UTR. H Dual-luciferase reporter assay revealing the PABPN1-mediated elongation effect on 3’UTR. I Western blot assay revealing the PABPN1-mediated gene silencing effect of miRNA. J Diagram showing vectors containing wild-type or mutant 3’UTRs of PABPN1 target genes. K Binding capacities of PABPN1 to wild-type or mutant 3’UTRs evaluated by RIP assays with anti-PABPN1 antibody. IgG was used as a negative control. Data are presented as the mean ± SD of three independent experiments. * P < 0.05