Fig. 1

Potential APA regulator PABPN1 is downregulated in BC cells. A Hierarchical clustering of samples (columns) in TCGA BLCA based on PDUI scores of each gene (rows). B Scatter plot showing expression levels of DEGs in subgroup A and C. C Volcano plot showing relative expression of genes in subgroup C to subgroup A. D Correlation coefficients between the expression of 40 potential APA regulators and the number of genes undergoing 3’UTR lengthening or shortening in samples from TCGA BLCA dataset. E A venn diagram of potential APA regulators and reported APA core factors. F PABPN1 protein levels in human normal urothelial epithelial cell line and BC cell lines. GAPDH was used as a loading control. G PABPN1 protein levels in BC tissues (T) and adjacent non-tumorous tissues (N). GAPDH was used as a loading control. H Relative expression of PABPN1 in low/high grade BC samples from TCGA. I Overall survival in patients from TCGA BLCA with PABPN1 low versus high expression. J Overall survival time (left axis, the height of dots), survival state, and PABPN1 expression (right axis, the height of bars) in patients from TCGA BLCA