Fig. 5

SEPT2-K74R inhibited cell migration and invasion in vivo A Mice in the SEPT2-K74R group gained the most weight. Huh7 stable cells were injected into the tail vein of NCG mice (8 mice each group). The mice were weighed every week (W) and were sacrificed 8 weeks after the injection. B Quantitative comparison of mice with lung metastases in each group. Fewer mice had lung metastasis in the SEPT2-K74R group. The red column indicates the mice had metastases. C Representative images of lung metastases in the tail vein injection model. After the mice were sacrificed, the lungs were dissected and photographed (upper panel). Blue arrows indicate lung metastases. Lung tissues were stained by H&E (bottom panel). D Quantitative comparison of the number of lung metastases in each group. E Quantitative comparison of mice with liver metastases in each group. The red column indicates the transferred mice. F Flowchart showing the orthotopic liver tumor implantation model. Huh7 stable cells were injected into the left lobe of the liver in NCG mice (8 mice per group). G Images of liver metastases in the orthotopic liver tumor implantation model. After the mice were sacrificed, the livers were dissected, photographed and weighed. Blue arrows indicate liver metastases. H Quantitative comparison of the number of liver metastases in each group. Mice in the SEPT2-K74R group had fewer liver metastases. I Representative images showing liver metastases in the orthotopic liver tumor implantation model. Tissues were stained by H&E. J, K Quantitative comparison of the number of mice with metastases in other lobes of the liver (J) and the number of mice with lung metastases (K). The red column indicates the transferred mice.The data are presented as the means ± SD. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001 (one-way ANOVA, two-way ANOVA). NC, negative control; WT, wild-type SEPT2; K74R, SEPT2-K74R mutant