Fig. 6

ASM deletion protects glomerular endothelial cell injury in LPS-AKI model. a and b Expression of acid sphingomyelinase (ASM) in renal cortex of ASM^+/− mice was detected by western blotting. c. CD31 immunohistochemical analysis in renal tissues. d-f Expression of neutrophil gelatinase-associated lipocalin (NGAL) and vascular cell adhesion molecule-1 (VCAM-1) in ASM^+/− mice and lipopolysaccharide (LPS)-acute kidney injury (AKI). g–i Western blot analysis of CD63 in kidney exosomes in the LPS-AKI model and CD63 immunohistochemical analysis in renal tissues. Data represent the mean ± SEM in independent experiments. ^*P < 0.05, ^**P < 0.01 versus Con or LPS group