Fig. 5

AMPKα represses the activation of NF-κB pathway. A Volcano plot showing the differential expression genes (DEGs) from DAOYs transfected with PRKAA1 siRNA or non-silencing control. B KEGG pathway enrichment of DEGs. C Heatmap of genes in Toll-like receptor signaling pathway. D GSEA enrichment plots of gene sets from Toll-like receptor signaling pathway and TNF-α signaling via NF-κB in the siPRKAA1 group. E RT-PCR detection of PRKAA1 and NF-κB target genes mRNAs in DAOYs transfected with PRKAA1 siRNA. F RT-PCR detection of PRKAA1 and NF-κB target genes in PRKAA1.^−/− DAOYs transfected with PRKAA1. G Western blot detection of phosphorylated AMPKα (P- AMPKα), IκBα, P65, and phosphorylated P65 (P-P65) in DAOYs. H RT-PCR detection of PRKAA1 and CCL5 mRNAs in DAOYs transfected with PRKAA1 siRNA. I Cell proliferation of DAOYs transfected with PRKAA1 or CCL5 siRNA were assessed by CCK-8. Migration J and invasion L abilities of DAOYs transfected with PRKAA1 or CCL5 siRNA evaluated by transwell assays with and without matrigel. K and M are quantification data of three independent experiments as in (J) and (L). Data in bar graphs are performed as mean ± SEM. * P < 0.05, ** P < 0.01, *** P < 0.001