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Fig. 6 | Cell & Bioscience

Fig. 6

From: Predictive biomarkers for the responsiveness of recurrent glioblastomas to activated killer cell immunotherapy

Fig. 6

Effects of three candidate genes on migration of NK cells toward GBM cancer cells. A Expression of TNFSF4, TNFRSF18, and IL12RB2 in T cells and U87MG cells was analyzed by flow cytometry. Left: Representative histogram plots. Gray histograms represent isotype controls. Right: Column graphs show the frequencies of TNFSF4+, TNFRSF18+, and IL12RB2+ cells. B Transwell migration assay: CFSE-stained NK cells were plated in the upper chamber of a Transwell insert with an 8-mm pore size. Cells were allowed to migrate for 8 h toward U87MG cells with or without T cells in the lower chamber. T cells were pre-incubated with blocking antibodies to TNFSF4, TNFRSF18, and IL12RB2 for 1 h. NK cells that migrated to the lower chamber were counted using a cell counter. The experiments were performed in triplicate

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