Fig. 6
From: Loss of TET2 impairs endothelial angiogenesis via downregulating STAT3 target genes
TET2 promotes angiogenesis in a STAT3-dependent manner. A, B, C Western blot of HUVECs infected with TET2 adenovirus in presence or absence of STAT3 siRNA under hypoxia D, F EdU assay of HUVECs infected with TET2 adenovirus in presence or absence of STAT3 siRNA under hypoxia. Scale bar, 50 μm. E Quantification of the mRNA expression levels of CCND1, MCL1 and HMOX1 in HUVECs infected with TET2 adenovirus in presence or absence of STAT3 siRNA under hypoxia. G, H Scratch assay and I, J Tube formation assay of HUVECs infected with TET2 adenovirus in presence or absence of STAT3 siRNA under hypoxia. Scale bar, 100 μm. n = 3–4 per group. B, C, F, H, J Two-way ANOVA with Bonferroni post hoc test. E One-way ANOVA with Bonferroni post hoc test. *, P < 0.05; **, P < 0.01