Fig. 5
From: Loss of TET2 impairs endothelial angiogenesis via downregulating STAT3 target genes
TET2 is recruited by STAT3 and activates its target genes. TET2-His and STAT3-Flag were ectopically expressed in HEK293T and the lysate of HEK293T was immunoprecipitated with A anti-His antibody or B anti-Flag antibody. C Schematic illustration of different plasmids of TET2 and STAT3. Different domains of TET2 and STAT3 were ectopically expressed in HEK293T and the lysate of HEK293T was immunoprecipitated with D anti-Flag antibody or E anti-HA antibody. F Heat map of related genes based on RNA sequencing. G The occupancy of TET2 at the promoter regions of STAT3-target genes was determined by ChIP-qPCR. H The site-specific 5-hmC and 5-mC levels were determined by using GluMS-qPCR. n = 3 per group. G, H One-way ANOVA with Bonferroni post hoc test. *, P < 0.05 vs. the STAT3 (−) and TET2 (−) group (5-hmC); #, P < 0.05 vs. the STAT3 (−) and TET2 (−) group (5-mC)