Fig. 3
From: Loss of TET2 impairs endothelial angiogenesis via downregulating STAT3 target genes
Deficiency of TET2 impairs blood flow recovery and density of capillary in the HLI model. A Genomic structure of TET2EC−KO mice. B The time points of treatments in mice. C, D The blood flow recovery of CDH5Cre; mTmG and TET2EC−KO; mTmG mice. E, F, G Representative hematoxylin–eosin (H & E) staining images and quantification of the necrotic area at the indicated times, as well as regenerating area 21 days after HLI. H, I Representative masson staining images at the indicated times and quantification of regenerating area 21 days after HLI. J, K Representative staining images of GFP-positive cells in the cross section of gastrocnemius muscles of the ligated hindlimb 14 days after HLI. GFP (green), DAPI (blue). L, M Flow cytometry analysis of GFP-positive cells in the gastrocnemius muscles (14 days). n = 5–7 mice per group. D, F, G, I, K Unpaired Student’s t‐test. M Two-way ANOVA with Bonferroni post hoc test. ns no significant; *, P < 0.05; **, P < 0.01; HLI hindlimb ischemia, E, H Scale bar, 20 μm. J Scar bar, 100 µm