Fig. 1
From: Loss of TET2 impairs endothelial angiogenesis via downregulating STAT3 target genes
Hypoxia impairs angiogenesis and downregulates TET2 expression in HUVECs. A, B EdU assay, C, D Scratch assay and E, F Tube formation assay of HUVECs under normoxia and hypoxia. G, H Dot blot assay of global 5-hmC and I ELISA assay of global 5-mC using genomic DNA from HUVECs treated with normoxia and hypoxia. J Quantification of the mRNA expression levels of TET1, TET2 and TET3 under normoxia and hypoxia. K, L Western blot of TET2 expression levels under hypoxia for 0 h, 24 h and 48 h. n = 3–4 per group. B, D, F, H, I, J Unpaired Student’s t‐test. K One-way ANOVA with Bonferroni post hoc test. ns no significant; *, P < 0.05; **, P < 0.01; Nx normoxia, Hx hypoxia. A Scale bar, 50 μm. C, E Scale bar, 100 μm