Fig. 5

Unc5b affected macrophage migration through p-CDC42/p-PAK pathway. The expression of CDC42, p-CDC42 A as well as PAK, p-PAK B in Raw 264.7 cells after ox-LDL stimulation for 0-120 min were analyzed by Western blotting; C Quantification of p-CDC42 and p-PAK expression from Western blotting. Data are reported as the mean ± S.D. (n＞3, **, p< 0.01vs vehicle group, *p< 0.05 vs vehicle group, t-test). D CDC42, p-CDC42 and Rac123 protein levels in Raw 264.7 cells were analyzed by Western blotting. E Western blot analysis of PAK and p-PAK protein levels in Raw 264.7 cells. F Raw 264.7 cells were pretreated with CDC42 inhibitor or PAK inhibitor, and then the number of cells on the dark side was identified by Transwell assay upon ox-LDL (50 μg/ml) treatment. Also the effects of CDC42 inhibitor or PAK inhibitor on the formation of macrophage pseudopodium was detected by F-actin staining (bar=20 μm, n=4). G Raw 264.7 cells were pretreated with Unc5b^siRNA or Unc5b^oe combined with ox-LDL (50 μg/ml) stimulation, the Unc5b, CDC42 and PAK protein levels were determined by Western blotting. H Atherosclerotic aortic sinus were collected from ApoE^−/− mice that was fed a Western diet (Development group) for 16 weeks, and also injected with Ad-NC, Ad-sh or Ad-oe for another 4 weeks. The aortic arch was stained for p-CDC42 and p-PAK (green).  Data are reported as the mean ± S.D. (n=4, *, p< 0.05 ; ***, p< 0.001; t-test.)