Fig. 4

Hypofucosylation of Unc5b regulated by Fut8 facilitates macrophage migration.  A, C The interaction between Unc5b and AAL, LCA, PHAL, VVL, MAL1, SNA detected by IP assays after ox-LDL treatment for 24 h. B, D Quantification of Unc5b expression from IP assay. Data are reported as the mean ± S.D. (n＞3, t-test). E HEK293T cells were pretreated with pCMV-Unc5b-GFP plasmid or pCMV-Unc5bko222,347-GFP plasmid and pCMV-Fut8-mCherry plasmid and cotransfected with pCMV-Fut8-mCherry plasmid, followed by immunostaining. ER (blue), Fut8 (red), Unc5b (green), merge (yellow)(bar=20 μm, n=4). F The α-1,6-fucosylated protein level of Unc5b was detected by LCA in cells with Fut8eo or Fut8siRNA plasmid treatment and the quantification of protein expression was shown below the blot. Data are reported as the mean ± S.D. (n＞3, *, p< 0.05vs NC group, t-test).