Fig. 1

Unc5b contributes to the formation of atherosclerotic plaques. A Representative Oil Red O-stained images of aortic valve sections; The intimal thickness of the aortic valve is shown by H&E staining; The aortic arch was stained with H&E to reveal the intimal thickness (bar=500 μm). B Quantitative image analysis of Figure A. C Serum lipid levels such as TC, TG, LDL-C, HDL-C in different groups. D Livers from ApoE^−/− mice in different group were stained with H&E, and the number of vacuolized structure was characterized as maker to present the level of liver lipid and necrosis. E Atherosclerotic aortic sinus were collected from ApoE^−/− mice and immunostaining assay was performed on the aortic to detect the expression of Unc5b as well as Fut8 in plaque (bar=200 μm, n=4). Unc5b (red), Fut8 (green), DAPI (blue); The content of macrophage (CD68⁺) in aortic valve plaques was determined by immunohistochemistry (bar=500 μm, n=4). F Quantification of Unc5b and Fut8 expression from immunostaining assay. Data are reported as the mean ± S.D. G Atherosclerotic aortic sinus were collected from ApoE^−/− mice in Development group, Ad-NC, Ad-sh and Ad-oe group. The aortic sinus was stained for Unc5b (red), Fut8 (green), CD68 (blue) and their co-localization (yellow merge). Data are reported as the mean ± S.D. (n＞3, ****, p< 0.0001vs vehicle group, ###, p< 0.001 vs Ad-NC group, t-test). The mice in the Ad-NC group, Ad-sh group and Ad-oe group were given NC or Unc5bshRNA or Unc5b over-expression adenovirus injection into the caudal vein for four weeks.