From: Comparison of DNA targeting CRISPR editors in human cells
Cas9 | Cas12a | Un1Cas12f1 | AsCas12f1 | |
---|---|---|---|---|
PAM | NGG | TTTV | TTTR | NTTR |
Size | SpCas9 (1368 aa) | AsCas12a (1307 aa); LbCas12a (1228 aa) | 529 aa | 422 aa |
Cutting site | -3th, -4th bp | 14th, 20th | 8th, 14th, and 25th | 14th, 20th |
Break site | Blunt end | Sticky end | Sticky end | Sticky end |
Specificity | + | +++ | +++ | +++ |
Activity | +++ | + | + | + |
CRISPR-based activation | +++ | +++ | +++ | - |
Application scenarios | Disrupt genes in cells or animal models | Disrupt genes for therapy purposes | Gene-activation-based therapy | Need to improve |