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Table 1 Comparison of the commonly used DNA targeting CRISPR editors

From: Comparison of DNA targeting CRISPR editors in human cells

 

Cas9

Cas12a

Un1Cas12f1

AsCas12f1

PAM

NGG

TTTV

TTTR

NTTR

Size

SpCas9 (1368 aa)

AsCas12a (1307 aa);

LbCas12a (1228 aa)

529 aa

422 aa

Cutting site

-3th, -4th bp

14th, 20th

8th, 14th, and 25th

14th, 20th

Break site

Blunt end

Sticky end

Sticky end

Sticky end

Specificity

+

+++

+++

+++

Activity

+++

+

+

+

CRISPR-based activation

+++

+++

+++

-

Application scenarios

Disrupt genes in cells or animal models

Disrupt genes for therapy purposes

Gene-activation-based therapy

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